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igfbp4 standards (R&D Systems)

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R&D Systems igfbp4 standards

Fig. 2. Expression levels of the igf1 (A), igf1r (B), HSF-1 (C), igfbp2 (D), igfbp3 (E), <t>igfbp4</t> (F), insr (G), lepr (H) and lif (I) genes in uterine samples from the FL1 (red) and FL2 (green) lines compared to those from the ctrl (blue) lines. The data are presented as the mean ± SEM (standard error of the mean) and were considered significant if p < 0.05 (Bonferroni posttest: * p < 0.05; ** p < 0.01; *** p < 0.005).

Igfbp4 Standards, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+igfbp4/Recombinant+Human+IGFBP-4+Protein%2C+CF/pm40158446-82-8-10
Average 93 stars, based on 15 article reviews

igfbp4 standards - by Bioz Stars, 2026-09

93/100 stars

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1) Product Images from "The special phenotypic characteristics of Dummerstorf superfertile mouse lines could depend on the expression levels of IGF-axis genes."

Article Title: The special phenotypic characteristics of Dummerstorf superfertile mouse lines could depend on the expression levels of IGF-axis genes.

Journal: Reproductive biology

doi: 10.1016/j.repbio.2025.101012

Fig. 2. Expression levels of the igf1 (A), igf1r (B), HSF-1 (C), igfbp2 (D), igfbp3 (E), igfbp4 (F), insr (G), lepr (H) and lif (I) genes in uterine samples from the FL1 (red) and FL2 (green) lines compared to those from the ctrl (blue) lines. The data are presented as the mean ± SEM (standard error of the mean) and were considered significant if p < 0.05 (Bonferroni posttest: * p < 0.05; ** p < 0.01; *** p < 0.005).

Figure Legend Snippet: Fig. 2. Expression levels of the igf1 (A), igf1r (B), HSF-1 (C), igfbp2 (D), igfbp3 (E), igfbp4 (F), insr (G), lepr (H) and lif (I) genes in uterine samples from the FL1 (red) and FL2 (green) lines compared to those from the ctrl (blue) lines. The data are presented as the mean ± SEM (standard error of the mean) and were considered significant if p < 0.05 (Bonferroni posttest: * p < 0.05; ** p < 0.01; *** p < 0.005).

Techniques Used: Expressing, IF-P

Fig. 4. Expression levels of the igf1 (A), hsf1 (B), igfbp2 (C), igfbp3 (D) and igfbp4 (E) genes in follicle samples from the FL1 (red) and FL2 (green) lines compared to those from the ctrl (blue) line. The data are presented as the mean ± SEM (standard error of the mean) and were considered significant if p < 0.05 (Bonferroni posttest: * p < 0.05; ** p < 0.01; *** p < 0.005).

Figure Legend Snippet: Fig. 4. Expression levels of the igf1 (A), hsf1 (B), igfbp2 (C), igfbp3 (D) and igfbp4 (E) genes in follicle samples from the FL1 (red) and FL2 (green) lines compared to those from the ctrl (blue) line. The data are presented as the mean ± SEM (standard error of the mean) and were considered significant if p < 0.05 (Bonferroni posttest: * p < 0.05; ** p < 0.01; *** p < 0.005).

Techniques Used: Expressing, IF-P

Fig. 3. Quantity of IGFBP2 (A), IGFBP3 (B) and IGFBP4 (C) binding to the IGF2 protein in uterus samples from the FL1 (red) and FL2 (green) lines compared to those from the ctrl (blue) line. This quantity has been measured using the enhanced chemiluminescence method, eliminating the background (Arbitrary Unit, AU). The data are presented as the mean ± SEM (standard error of the mean) and were considered significant if p < 0.05 (Bonferroni posttest: * p < 0.05; ** p < 0.01; *** p < 0.005).

Figure Legend Snippet: Fig. 3. Quantity of IGFBP2 (A), IGFBP3 (B) and IGFBP4 (C) binding to the IGF2 protein in uterus samples from the FL1 (red) and FL2 (green) lines compared to those from the ctrl (blue) line. This quantity has been measured using the enhanced chemiluminescence method, eliminating the background (Arbitrary Unit, AU). The data are presented as the mean ± SEM (standard error of the mean) and were considered significant if p < 0.05 (Bonferroni posttest: * p < 0.05; ** p < 0.01; *** p < 0.005).

Techniques Used: Binding Assay, IF-P

Fig. 6. Expression levels of igfbp2 (A), igfbp3 (B) and igfbp4 (C) in liver samples from the FL1 (red) and FL2 (green) lines compared to those from the ctrl (blue) line. The data are presented as the mean ± SEM (standard error of the mean) and were considered significant if p < 0.05 (Bonferroni posttest: * p < 0.05; ** p < 0.01; *** p < 0.005). The outliers were analyzed by the ROUT method using GraphPad and excluded.

Figure Legend Snippet: Fig. 6. Expression levels of igfbp2 (A), igfbp3 (B) and igfbp4 (C) in liver samples from the FL1 (red) and FL2 (green) lines compared to those from the ctrl (blue) line. The data are presented as the mean ± SEM (standard error of the mean) and were considered significant if p < 0.05 (Bonferroni posttest: * p < 0.05; ** p < 0.01; *** p < 0.005). The outliers were analyzed by the ROUT method using GraphPad and excluded.

Techniques Used: Expressing, IF-P

Recombinant:

Article Title: The pro-atherogenic enzyme PAPP-A is active in eluates from human carotid and femoral atherosclerotic plaques
Article Snippet: .. Recombinant human IGFBP4 from RnD Systems (Cat# 804-GB-025) and IGF1 from Austral Biologicals (Cat# GF-050-8) were pre-incubated in media/PBS? and added to all conditioned media from different plaque?-incubations (200 μL) in a final concentration of 1200 ng/mL (of both IGF1 and IGFBP4). ..

Article Title: Structure of the PAPP-A BP5 complex reveals mechanism of substrate recognition
Article Snippet: The target fractions were concentrated using 10 kDa MWCO concentrator column (Millipore UFC901024) and stored at −80 °C until further use. .. Recombinant human IGFBP4 (R&D Systems, Cat. no. 804-GB-025) was reconstituted in 1X PBS. .. Recombinant human IGF1 was sourced from Abcam (Ab270062) as lyophilized aliquots and reconstituted in 1X PBS, then aliquoted and stored at −80 °C.

Article Title: Structure of the PAPP-A BP5 complex reveals mechanism of substrate recognition.
Article Snippet: The target fractions were concentrated using 10 kDa MWCO concentrator column (Millipore UFC901024) and stored at −80 °C until further use. .. Recombinant human IGFBP4 (R&D Systems, Cat. no. 804-GB-025) was reconstituted in 1X PBS. .. Recombinant human IGF1 was sourced from Abcam (Ab270062) as lyophilized aliquots and reconstituted in 1X PBS, then aliquoted and stored at −80 °C.

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Journal: Reproductive biology

Article Title: The special phenotypic characteristics of Dummerstorf superfertile mouse lines could depend on the expression levels of IGF-axis genes.

doi: 10.1016/j.repbio.2025.101012

Figure Lengend Snippet: Fig. 2. Expression levels of the igf1 (A), igf1r (B), HSF-1 (C), igfbp2 (D), igfbp3 (E), igfbp4 (F), insr (G), lepr (H) and lif (I) genes in uterine samples from the FL1 (red) and FL2 (green) lines compared to those from the ctrl (blue) lines. The data are presented as the mean ± SEM (standard error of the mean) and were considered significant if p < 0.05 (Bonferroni posttest: * p < 0.05; ** p < 0.01; *** p < 0.005).

Article Snippet: Serial dilutions of recombinant human IGFBP2, IGFBP3 and IGFBP4 standards (R&D Systems, Wiesbaden, Germany) in artificial serum matrix (Biopanda, County Down, United Kingdom) were subjected to the same treatment.

Techniques: Expressing, IF-P

Journal: Reproductive biology

Article Title: The special phenotypic characteristics of Dummerstorf superfertile mouse lines could depend on the expression levels of IGF-axis genes.

doi: 10.1016/j.repbio.2025.101012

Figure Lengend Snippet: Fig. 4. Expression levels of the igf1 (A), hsf1 (B), igfbp2 (C), igfbp3 (D) and igfbp4 (E) genes in follicle samples from the FL1 (red) and FL2 (green) lines compared to those from the ctrl (blue) line. The data are presented as the mean ± SEM (standard error of the mean) and were considered significant if p < 0.05 (Bonferroni posttest: * p < 0.05; ** p < 0.01; *** p < 0.005).

Techniques: Expressing, IF-P

Journal: Reproductive biology

Article Title: The special phenotypic characteristics of Dummerstorf superfertile mouse lines could depend on the expression levels of IGF-axis genes.

doi: 10.1016/j.repbio.2025.101012

Figure Lengend Snippet: Fig. 3. Quantity of IGFBP2 (A), IGFBP3 (B) and IGFBP4 (C) binding to the IGF2 protein in uterus samples from the FL1 (red) and FL2 (green) lines compared to those from the ctrl (blue) line. This quantity has been measured using the enhanced chemiluminescence method, eliminating the background (Arbitrary Unit, AU). The data are presented as the mean ± SEM (standard error of the mean) and were considered significant if p < 0.05 (Bonferroni posttest: * p < 0.05; ** p < 0.01; *** p < 0.005).

Techniques: Binding Assay, IF-P

Journal: Reproductive biology

Article Title: The special phenotypic characteristics of Dummerstorf superfertile mouse lines could depend on the expression levels of IGF-axis genes.

doi: 10.1016/j.repbio.2025.101012

Figure Lengend Snippet: Fig. 6. Expression levels of igfbp2 (A), igfbp3 (B) and igfbp4 (C) in liver samples from the FL1 (red) and FL2 (green) lines compared to those from the ctrl (blue) line. The data are presented as the mean ± SEM (standard error of the mean) and were considered significant if p < 0.05 (Bonferroni posttest: * p < 0.05; ** p < 0.01; *** p < 0.005). The outliers were analyzed by the ROUT method using GraphPad and excluded.

Techniques: Expressing, IF-P

Expression pattern of components of the STC2 - PAPP-A - IGFBP4 - IGF1 axis. a. Carotid plaques stained by masson trichrome and immunohistochemistry for STC2, PAPP-A, IGFBP4, and IGF1R. Arrowheads point to examples of stained cells. b. Single-cell RNA sequencing data from Wirka et al., 2019, displayed as UMAPs showing annotated cell populations, and expression pattern of PAPP-A , STC2 , IGFBP4 , IGF1R , and IGF1 . SMC = smooth muscle cell; mSMC = modulated SMC; EC = endothelial cell.

Journal: Atherosclerosis Plus

Article Title: The pro-atherogenic enzyme PAPP-A is active in eluates from human carotid and femoral atherosclerotic plaques

doi: 10.1016/j.athplu.2024.09.001

Figure Lengend Snippet: Expression pattern of components of the STC2 - PAPP-A - IGFBP4 - IGF1 axis. a. Carotid plaques stained by masson trichrome and immunohistochemistry for STC2, PAPP-A, IGFBP4, and IGF1R. Arrowheads point to examples of stained cells. b. Single-cell RNA sequencing data from Wirka et al., 2019, displayed as UMAPs showing annotated cell populations, and expression pattern of PAPP-A , STC2 , IGFBP4 , IGF1R , and IGF1 . SMC = smooth muscle cell; mSMC = modulated SMC; EC = endothelial cell.

Article Snippet: Recombinant human IGFBP4 from RnD Systems (Cat# 804-GB-025) and IGF1 from Austral Biologicals (Cat# GF-050-8) were pre-incubated in media/PBS? and added to all conditioned media from different plaque?-incubations (200 μL) in a final concentration of 1200 ng/mL (of both IGF1 and IGFBP4).

Techniques: Expressing, Staining, Immunohistochemistry, RNA Sequencing

Proteolytically active PAPP-A is present in atherosclerotic plaques. a. Study design: Plaque samples from carotid and femoral arteries were incubated in culture media for 24 h, and conditioned media was harvested for analyses. b-c. PAPP-A (b) and STC2 (c) concentration in conditioned media after 24 h of incubation quantified by ELISA. d. Principle of PAPP-A activity assay: Recombinant IGFBP4:IGF1 complex (32 kDa) is incubated with sample containing active PAPP-A resulting in proteolytic cleavage to 14 kDa IGFBP4 fragments. e. Conditioned media from each of the 20 plaque samples were incubated with recombinant IGFBP4:IGF1 for 0 or 24 h and analysed for IGFBP4 cleavage by Western blotting. In all samples, no IGFBP4 fragments are detected at the 0-h time point, but IGFBP4 fragments emerges after 24 h. No PAPP-A activity was detected in the media without tissue incubation (media 24 h). f. Quantitation of PAPP-A activity based on Western blotting. g. Correlation between PAPP-A concentration and -activity. h. Correlation between PAPP-A:STC2 molar ratio and PAPP-A activity. i. PAPP-A:STC2 molar ratio in conditioned media.

Journal: Atherosclerosis Plus

Article Title: The pro-atherogenic enzyme PAPP-A is active in eluates from human carotid and femoral atherosclerotic plaques

doi: 10.1016/j.athplu.2024.09.001

Figure Lengend Snippet: Proteolytically active PAPP-A is present in atherosclerotic plaques. a. Study design: Plaque samples from carotid and femoral arteries were incubated in culture media for 24 h, and conditioned media was harvested for analyses. b-c. PAPP-A (b) and STC2 (c) concentration in conditioned media after 24 h of incubation quantified by ELISA. d. Principle of PAPP-A activity assay: Recombinant IGFBP4:IGF1 complex (32 kDa) is incubated with sample containing active PAPP-A resulting in proteolytic cleavage to 14 kDa IGFBP4 fragments. e. Conditioned media from each of the 20 plaque samples were incubated with recombinant IGFBP4:IGF1 for 0 or 24 h and analysed for IGFBP4 cleavage by Western blotting. In all samples, no IGFBP4 fragments are detected at the 0-h time point, but IGFBP4 fragments emerges after 24 h. No PAPP-A activity was detected in the media without tissue incubation (media 24 h). f. Quantitation of PAPP-A activity based on Western blotting. g. Correlation between PAPP-A concentration and -activity. h. Correlation between PAPP-A:STC2 molar ratio and PAPP-A activity. i. PAPP-A:STC2 molar ratio in conditioned media.

Techniques: Incubation, Concentration Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Recombinant, Western Blot, Quantitation Assay

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igfbp4 standards (R&D Systems)

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1) Product Images from "The special phenotypic characteristics of Dummerstorf superfertile mouse lines could depend on the expression levels of IGF-axis genes."

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